![]() Therefore, this novel system could be utilized as a safe and efficient GLP-1 gene delivery system for type 2 diabetes therapy. When transfection medium containing secreted GLP-1 was transferred to NIT-1 insulinoma cells, the highest secretion level of insulin was induced in ABP/TSTA (SP-GLP-1) polyplex medium-treated cells. Additionally, 2.5–3.5 folds increased level of GLP-1 secretion was found in ABP gene carrier system in comparison with PEI25k. It was also observed in GLP-1 ELISA that GLP-1 secretion level of TSTA (SP-GLP-1) pDNA system was 2.7–3.4 times higher than those of pβ-GLP-1 and 1.5–1.7 times than TSTA (GLP-1). ![]() It was found in RT-PCR experiments that TSTA-SP pDNA systems showed increased GLP-1 gene transcription level in comparison with mono pDNA system (pβ-GLP-1). ABP showed no cytotoxicity contrary to PEI25k. ![]() Their polyplexes have about 140 nm-sizes and 20 mV Zeta-potential values. Its potential for GLP-1 gene delivery system was investigated with employment of arginine- grafted bioreducible polymer (ABP) as a gene carrier. ![]() Glucagon-like peptide (GLP-1) encoding dual plasmid (pDNA) system (TSTA (SP-GLP-1)) which is composed of pβ-Gal4-p65 and pUAS-SP-GLP-1 was constructed to improve the production and secretion of expressed GLP-1 by combining the advantages of signal peptide (SP) and two-step transcription amplification (TSTA) system. ![]()
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![]() Step 5: Start playing the song of a music playlist or browse for a piece of favorite song music, it will get automatically recorded. Step 4: Now sign in to your YouTube account. Step 3: Select YouTube Music as the source of music Step 1: After launching AudiCable Audio Recorder, click the Settings icon on the upper right corner and choose the output format ![]() How to Use AudiCable Audio Recorder to Record YouTube Music:
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